The Playbook for Urban Biodiversity is a comprehensive guide developed by the University of Melbourne to support biodiversity conservation and enhancement in urban environments. Drawing on extensive research and case studies, the playbook provides practical strategies for integrating biodiversity considerations into urban planning, design, and management. It addresses the ecological, social, and economic benefits of urban biodiversity while offering actionable guidance for practitioners, policymakers, and community groups. The resource emphasizes evidence-based approaches and includes tools for assessing, monitoring, and improving biodiversity outcomes in cities across Australia.